› 1mlz

X-ray diffraction
2.15Å resolution

Crystal Structure of 7,8-Diaminopelargonic Acid Synthase in complex with the trans-isomer of amiclenomycin.

Released:
DOI: 10.2210/pdb1mlz/pdb
PDB entry 1mlz coloured by chain, front view.
PDB entry 1mlz coloured by chain, side view.
PDB entry 1mlz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for the inhibition of the biosynthesis of biotin by the antibiotic amiclenomycin.
Sandmark J, Mann S, Marquet A, Schneider G
J Biol Chem 277 43352-8 (2002)
PMID: 12218056

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 47.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12995 (Residues: 1-429; Coverage: 100%)
Gene names: JW0757, b0774, bioA
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand TZA 2 x TZA
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2
Unit cell:
a: 128.034Å b: 55.96Å c: 116.001Å
α: 90° β: 109.65° γ: 90°
R-values:
R R work R free
0.207 0.205 0.24
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Biotin biosynthesis in Mycobacterium tuberculosis: physiology, biochemistry and molecular intervention.
Salaemae et al. (2011)
2 more