› 1mow

X-ray diffraction
2.4Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114196 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI; Homing endonuclease I-DmoI Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 260 amino acids
Theoretical weight: 30.03 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P21505 (Residues: 1-102, 108-108; Coverage: 53%)
  • Canonical: P05725 (Residues: 9-10, 12-163; Coverage: 95%)
Sequence domains:
Structure domains: Homing endonucleases
5'-D(*CP*CP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*AP*GP*TP*TP*CP*CP*GP*GP*CP*G)-3' Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 23 nucleotides
Theoretical weight: 7 KDa
5'-D(*CP*GP*CP*CP*GP*GP*AP*AP*CP*TP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP*GP*G)-3' Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 23 nucleotides
Theoretical weight: 7.12 KDa

Ligands and Environments

3 bound ligands:
Ligand GOL 18 x GOL
Ligand MG 6 x MG
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P31
Unit cell:
a: 131.76Å b: 131.76Å c: 120.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.231 0.257
Expression system: Escherichia coli

Quick links

Citations

42 review citations

Genome editing. The new frontier of genome engineering with CRISPR-Cas9.
Doudna et al. (2014)
41 more

4 mentions without citation

G protein betagamma subunits as targets for small molecule therapeutic development.
Smrcka et al. (2008)
3 more