› 1mru

X-ray diffraction
3Å resolution

Intracellular Ser/Thr protein kinase domain of Mycobacterium tuberculosis PknB.

Released:
DOI: 10.2210/pdb1mru/pdb
PDB entry 1mru coloured by chain, front view.
PDB entry 1mru coloured by chain, side view.
PDB entry 1mru coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure of Mycobacterium tuberculosis PknB supports a universal activation mechanism for Ser/Thr protein kinases.
Young TA, Delagoutte B, Endrizzi JA, Falick AM, Alber T
Nat Struct Biol 10 168-74 (2003)
PMID: 12548283

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknB Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 33.52 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WI81 (Residues: 1-308; Coverage: 49%)
Gene names: MTCY10H4.14c, Rv0014c, pknB
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand AGS 2 x AGS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 63.1Å b: 50.17Å c: 111.07Å
α: 90° β: 96.19° γ: 90°
R-values:
R R work R free
0.235 0.232 0.297
Expression system: Escherichia coli

Quick links

Citations

29 review citations

The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis.
Sauvage et al. (2008)
28 more

20 mentions without citation

Eukaryote-like serine/threonine kinases and phosphatases in bacteria.
Pereira et al. (2011)
19 more