› 1mvn

X-ray diffraction
2.21Å resolution

PPC decarboxylase mutant C175S complexed with pantothenoylaminoethenethiol

Released:
DOI: 10.2210/pdb1mvn/pdb
PDB entry 1mvn coloured by chain, front view.
PDB entry 1mvn coloured by chain, side view.
PDB entry 1mvn coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structure of the plant PPC decarboxylase AtHAL3a complexed with an ene-thiol reaction intermediate.
Steinbacher S, Hernández-Acosta P, Bieseler B, Blaesse M, Huber R, Culiáñez-Macià FA, Kupke T
J Mol Biol 327 193-202 (2003)
PMID: 12614618

Function and Biology Details

Reaction catalysed: 
N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopantothenoylcysteine decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.36 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SWE5 (Residues: 1-209; Coverage: 100%)
Gene names: At3g18030, COAC1, HAL3A, MBG14.2
Sequence domains: Flavoprotein
Structure domains: Flavin prenyltransferase-like

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
1 bound ligand:
Ligand PCO 1 x PCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P63
Unit cell:
a: 111.181Å b: 111.181Å c: 33.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.21 0.264
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes.
Repka et al. (2017)
5 more

3 mentions without citation

Crystal structure of UbiX, an aromatic acid decarboxylase from the psychrophilic bacterium Colwellia psychrerythraea that undergoes FMN-induced conformational changes.
Do et al. (2015)
2 more