1mvr

Electron Microscopy
12.8Å resolution

Decoding Center & Peptidyl transferase center from the X-ray structure of the Thermus thermophilus 70S ribosome, aligned to the low resolution Cryo-EM map of E.coli 70S Ribosome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-151452 (preferred)
Entry contents:
2 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (8 distinct):
Small ribosomal subunit protein uS12 Chain: O
Molecule details ›
Chain: O
Length: 135 amino acids
Theoretical weight: 14.92 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Sequence domains: Ribosomal protein S12/S23
Large ribosomal subunit protein uL11 Chain: L
Molecule details ›
Chain: L
Length: 140 amino acids
Theoretical weight: 14.98 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P29395 (Residues: 2-141; Coverage: 99%)
Gene names: TM_0454, rplK
Sequence domains:
mRNA, triplet codon (A-site) Chain: 1
Molecule details ›
Chain: 1
Length: 3 nucleotides
Theoretical weight: 874 Da
Helix 34 of 16S rRNA Chain: A
Molecule details ›
Chain: A
Length: 45 nucleotides
Theoretical weight: 14.47 KDa
Helix 44 of 16S rRNA Chain: B
Molecule details ›
Chain: B
Length: 96 nucleotides
Theoretical weight: 31.21 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
Helix 69 of 23S rRNA Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 6.08 KDa
Helix 89 of 23S rRNA Chain: D
Molecule details ›
Chain: D
Length: 59 nucleotides
Theoretical weight: 19 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
Helix 93 of 23S rRNA Chain: E
Molecule details ›
Chain: E
Length: 27 nucleotides
Theoretical weight: 8.71 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 12.8Å
Relevant EMDB volumes: EMD-1006, EMD-1007, EMD-1008, EMD-1009, EMD-1010