› 1mwe

X-ray diffraction
1.7Å resolution

THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH SIALIC ACID AT 4 DEGREES C REVEALING A SECOND SIALIC ACID BINDING SITE

Released:
DOI: 10.2210/pdb1mwe/pdb
PDB entry 1mwe coloured by chain, front view.
PDB entry 1mwe coloured by chain, side view.
PDB entry 1mwe coloured by chain, top view.
Source organism: Influenza A virus
Primary publication:
Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases.
Varghese JN, Colman PM, van Donkelaar A, Blick TJ, Sahasrabudhe A, McKimm-Breschkin JL
Proc Natl Acad Sci U S A 94 11808-12 (1997)
PMID: 9342319

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.72 KDa
Source organism: Influenza A virus
UniProt:
  • Canonical: P03472 (Residues: 83-470; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 3 x NAG
Ligand SIA 2 x SIA
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE M18X
Spacegroup: I432
Unit cell:
a: 181.04Å b: 181.04Å c: 181.04Å
α: 90° β: 90° γ: 90°

Quick links

Citations

9 review citations

Sialic Acid Receptors of Viruses.
Matrosovich et al. (2015)
8 more

24 mentions without citation

Influenza Virus Neuraminidase Structure and Functions.
McAuley et al. (2019)
23 more