› 1mwh

X-ray diffraction
2.5Å resolution

REOVIRUS POLYMERASE LAMBDA3 BOUND TO MRNA CAP ANALOG

Released:
DOI: 10.2210/pdb1mwh/pdb
PDB entry 1mwh coloured by chain, front view.
PDB entry 1mwh coloured by chain, side view.
PDB entry 1mwh coloured by chain, top view.
Source organism: Reovirus sp.
Primary publication:
RNA synthesis in a cage--structural studies of reovirus polymerase lambda3.
Tao Y, Farsetta DL, Nibert ML, Harrison SC
Cell 111 733-45 (2002)
PMID: 12464184

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase lambda-3 Chain: A
Molecule details ›
Chain: A
Length: 1267 amino acids
Theoretical weight: 142.42 KDa
Source organism: Reovirus sp.
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0CK31 (Residues: 1-1267; Coverage: 100%)
Gene name: L1
Sequence domains: Reovirus RNA-dependent RNA polymerase lambda 3
Structure domains: RNA-directed RNA polymerase lambda-3

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand GTG 1 x GTG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 70.891Å b: 84.944Å c: 249.246Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.209 0.245
Expression system: Spodoptera frugiperda

Quick links

Citations

48 review citations

Influenza virus RNA polymerase: insights into the mechanisms of viral RNA synthesis.
Te Velthuis et al. (2016)
47 more

6 mentions without citation

RNA Dependent RNA Polymerases: Insights from Structure, Function and Evolution.
Venkataraman et al. (2018)
5 more