› 1mwi

X-ray diffraction
2.35Å resolution

Crystal structure of a MUG-DNA product complex

Released:
DOI: 10.2210/pdb1mwi/pdb
PDB entry 1mwi coloured by chain, front view.
PDB entry 1mwi coloured by chain, side view.
PDB entry 1mwi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions.
Barrett TE, Savva R, Panayotou G, Barlow T, Brown T, Jiricny J, Pearl LH
Cell 92 117-29 (1998)
PMID: 9489705

Function and Biology Details

Reaction catalysed: 
Specifically hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114209 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
G/U mismatch-specific DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 18.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A9H1 (Residues: 1-168; Coverage: 100%)
Gene names: JW3040, b3068, mug, ygjF
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain
5'-D(*CP*GP*CP*GP*AP*GP*(AAB)P*TP*CP*GP*CP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.57 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P42212
Unit cell:
a: 101.35Å b: 101.35Å c: 45.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.21 0.28
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

28 review citations

Uracil in DNA--occurrence, consequences and repair.
Krokan et al. (2002)
27 more

7 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
6 more