› 1n07

X-ray diffraction
2.45Å resolution

Crystal Structure of Schizosaccharomyces pombe Riboflavin Kinase Reveals a Novel ATP and Riboflavin Binding Fold

Released:
DOI: 10.2210/pdb1n07/pdb
PDB entry 1n07 coloured by chain, front view.
PDB entry 1n07 coloured by chain, side view.
PDB entry 1n07 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold.
Bauer S, Kemter K, Bacher A, Huber R, Fischer M, Steinbacher S
J Mol Biol 326 1463-73 (2003)
PMID: 12595258

Function and Biology Details

Reaction catalysed: 
ATP + riboflavin = ADP + FMN
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.94 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O74866 (Residues: 1-163; Coverage: 100%)
Gene names: SPCC18.16c, fmn1
Sequence domains: Riboflavin kinase
Structure domains: Riboflavin kinase-like

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
1 bound ligand:
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 38.807Å b: 45.767Å c: 51.895Å
α: 90.69° β: 111.03° γ: 97.31°
R-values:
R R work R free
0.231 0.228 0.285
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
Abbas et al. (2011)
4 more

7 mentions without citation

Structure of the herpesvirus major capsid protein.
Bowman et al. (2003)
6 more