› 1n08

X-ray diffraction
1.6Å resolution

Crystal Structure of Schizosaccharomyces pombe Riboflavin Kinase Reveals a Novel ATP and Riboflavin Binding Fold

Released:
DOI: 10.2210/pdb1n08/pdb
PDB entry 1n08 coloured by chain, front view.
PDB entry 1n08 coloured by chain, side view.
PDB entry 1n08 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold.
Bauer S, Kemter K, Bacher A, Huber R, Fischer M, Steinbacher S
J Mol Biol 326 1463-73 (2003)
PMID: 12595258

Function and Biology Details

Reaction catalysed: 
ATP + riboflavin = ADP + FMN
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.94 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O74866 (Residues: 1-163; Coverage: 100%)
Gene names: SPCC18.16c, fmn1
Sequence domains: Riboflavin kinase
Structure domains: Riboflavin kinase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 41.235Å b: 45.321Å c: 52.849Å
α: 89.7° β: 112.04° γ: 98.41°
R-values:
R R work R free
0.224 0.22 0.252
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
Abbas et al. (2011)
4 more

5 mentions without citation

Reciprocal carbonyl-carbonyl interactions in small molecules and proteins.
Rahim et al. (2017)
4 more