› 1n3f

X-ray diffraction
2Å resolution

Crystal structure of I-CreI bound to a palindromic DNA sequence II (palindrome of right side of wildtype DNA target sequence)

Released:
DOI: 10.2210/pdb1n3f/pdb
PDB entry 1n3f coloured by chain, front view.
PDB entry 1n3f coloured by chain, side view.
PDB entry 1n3f coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI.
Chevalier B, Turmel M, Lemieux C, Monnat RJ, Stoddard BL
J Mol Biol 329 253-69 (2003)
PMID: 12758074

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114227 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 163 amino acids
Theoretical weight: 18.72 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 1-163; Coverage: 100%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*GP*A)-3' Chains: C, E, I, K
Molecule details ›
Chains: C, E, I, K
Length: 14 nucleotides
Theoretical weight: 4.26 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
5'-D(P*GP*AP*CP*AP*GP*TP*TP*TP*CP*G-3') Chains: D, F, J, L
Molecule details ›
Chains: D, F, J, L
Length: 10 nucleotides
Theoretical weight: 3.06 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 78.32Å b: 76.39Å c: 81.06Å
α: 90° β: 108.75° γ: 90°
R-values:
R R work R free
0.195 0.195 0.228
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.
Silva et al. (2011)
8 more

1 mention without citation

Small local variations in B-form DNA lead to a large variety of global geometries which can accommodate most DNA-binding protein motifs.
Marathe et al. (2009)