› 1n5o

X-ray diffraction
2.8Å resolution

Structural consequences of a cancer-causing BRCA1-BRCT missense mutation

Released:
DOI: 10.2210/pdb1n5o/pdb
PDB entry 1n5o coloured by chain, front view.
PDB entry 1n5o coloured by chain, side view.
PDB entry 1n5o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural consequences of a cancer-causing BRCA1-BRCT missense mutation.
Williams RS, Glover JN
J Biol Chem 278 2630-5 (2003)
PMID: 12427738

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Breast cancer type 1 susceptibility protein Chain: X
Molecule details ›
Chain: X
Length: 214 amino acids
Theoretical weight: 24.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P38398 (Residues: 1646-1859; Coverage: 12%)
Gene names: BRCA1, RNF53
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain

Ligands and Environments

2 bound ligands:
Ligand CO 1 x CO
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 114.535Å b: 114.535Å c: 121.527Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.275 0.274 0.298
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Homologous recombination and human health: the roles of BRCA1, BRCA2, and associated proteins.
Prakash et al. (2015)
13 more

8 mentions without citation

Fanconi anemia pathway as a prospective target for cancer intervention.
Liu et al. (2020)
7 more