› 1n69

X-ray diffraction
2.2Å resolution

Crystal structure of human saposin B

Released:
DOI: 10.2210/pdb1n69/pdb
PDB entry 1n69 coloured by chain, front view.
PDB entry 1n69 coloured by chain, side view.
PDB entry 1n69 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of saposin B reveals a dimeric shell for lipid binding.
Ahn VE, Faull KF, Whitelegge JP, Fluharty AL, Privé GG
Proc Natl Acad Sci U S A 100 38-43 (2003)
PMID: 12518053

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Saposin-B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 81 amino acids
Theoretical weight: 9.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07602 (Residues: 195-273; Coverage: 16%)
Gene names: GLBA, PSAP, SAP1
Sequence domains: Saposin-like type B, region 2
Structure domains: Saposin-like

Ligands and Environments

1 bound ligand:
Ligand 3PE 1 x 3PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P3121
Unit cell:
a: 72.14Å b: 72.14Å c: 94.366Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.222 0.262
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Principles of lysosomal membrane digestion: stimulation of sphingolipid degradation by sphingolipid activator proteins and anionic lysosomal lipids.
Kolter et al. (2005)
26 more

17 mentions without citation

A short guided tour through functional and structural features of saposin-like proteins.
Bruhn H. (2005)
16 more