› 1n7a

X-ray diffraction
1.2Å resolution

RIP-Radiation-damage Induced Phasing

Released:
DOI: 10.2210/pdb1n7a/pdb
PDB entry 1n7a coloured by chain, front view.
PDB entry 1n7a coloured by chain, side view.
PDB entry 1n7a coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Specific radiation damage can be used to solve macromolecular crystal structures.
Ravelli RB, Leiros HK, Pan B, Caffrey M, McSweeney S
Structure 11 217-24 (2003)
PMID: 12575941

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113083 (preferred)
Entry contents:
1 distinct DNA/RNA hybrid molecule
Macromolecule:
RNA/DNA (5'-R(*U)-D(P*(BGM))-R(P*AP*GP*GP*U)-3') Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 6 nucleotides
Theoretical weight: 2 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand K 9 x K
Ligand SPM 2 x SPM
1 modified residue:
Ligand BGM 8 x BGM

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 31.233Å b: 51.102Å c: 70.651Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.124 0.156
Expression system: Not provided

Quick links

Citations

9 review citations

Membrane protein crystallization.
Caffrey M. (2003)
8 more

1 mention without citation

ElTetrado: a tool for identification and classification of tetrads and quadruplexes.
Zok et al. (2020)