› 1n8z

X-ray diffraction
2.52Å resolution

Crystal structure of extracellular domain of human HER2 complexed with Herceptin Fab

Released:
DOI: 10.2210/pdb1n8z/pdb
PDB entry 1n8z coloured by chain, front view.
PDB entry 1n8z coloured by chain, side view.
PDB entry 1n8z coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the extracellular region of HER2 alone and in complex with the Herceptin Fab.
Cho HS, Mason K, Ramyar KX, Stanley AM, Gabelli SB, Denney DW, Leahy DJ
Nature 421 756-60 (2003)
PMID: 12610629

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226715 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Herceptin Fab (antibody) - light chain Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.47 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Herceptin Fab (antibody) - heavy chain Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 23.43 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Receptor tyrosine-protein kinase erbB-2 Chain: C
Molecule details ›
Chain: C
Length: 607 amino acids
Theoretical weight: 66.99 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04626 (Residues: 23-629; Coverage: 49%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 66.17Å b: 109.77Å c: 175.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.222 0.284
Expression systems:
  • Not provided
  • Cricetulus griseus

Quick links

Citations

243 review citations

ERBB receptors and cancer: the complexity of targeted inhibitors.
Hynes et al. (2005)
242 more

176 mentions without citation

The role of HER2 in cancer therapy and targeted drug delivery.
Tai et al. (2010)
175 more