› 1nbu

X-ray diffraction
1.6Å resolution

7,8-Dihydroneopterin Aldolase Complexed with Product From Mycobacterium Tuberculosis

Released:
DOI: 10.2210/pdb1nbu/pdb
PDB entry 1nbu coloured by chain, front view.
PDB entry 1nbu coloured by chain, side view.
PDB entry 1nbu coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Regulation by oligomerization in a mycobacterial folate biosynthetic enzyme.
Goulding CW, Apostol MI, Sawaya MR, Phillips M, Parseghian A, Eisenberg D
J Mol Biol 349 61-72 (2005)
PMID: 15876368

Function and Biology Details

Reactions catalysed: 
7,8-dihydroneopterin + O(2) = 7,8-dihydroxanthopterin + formate + glycolaldehyde
7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
7,8-dihydroneopterin = 7,8-dihydromonapterin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161848 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Dihydroneopterin aldolase Chains: A, E, F, G, H
Molecule details ›
Chains: A, E, F, G, H
Length: 119 amino acids
Theoretical weight: 13.25 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNC5 (Residues: 1-119; Coverage: 90%)
Gene names: MTCY07H7B.15, Rv3607c, folB
Sequence domains: Dihydroneopterin aldolase
Structure domains: GTP Cyclohydrolase I, domain 2
Dihydroneopterin aldolase Chains: B, D
Molecule details ›
Chains: B, D
Length: 119 amino acids
Theoretical weight: 13.21 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WNC5 (Residues: 1-119; Coverage: 90%)
Gene names: MTCY07H7B.15, Rv3607c, folB
Sequence domains: Dihydroneopterin aldolase
Structure domains: GTP Cyclohydrolase I, domain 2
Dihydroneopterin aldolase Chain: C
Molecule details ›
Chain: C
Length: 119 amino acids
Theoretical weight: 13.12 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WNC5 (Residues: 1-119; Coverage: 90%)
Gene names: MTCY07H7B.15, Rv3607c, folB
Sequence domains: Dihydroneopterin aldolase
Structure domains: GTP Cyclohydrolase I, domain 2

Ligands and Environments

1 bound ligand:
Ligand PH2 8 x PH2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 78.56Å b: 85.15Å c: 74.79Å
α: 90° β: 112.69° γ: 90°
R-values:
R R work R free
0.177 0.166 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

8 review citations

Mycobacterium tuberculosis folate metabolism and the mechanistic basis for para-aminosalicylic acid susceptibility and resistance.
Minato et al. (2015)
7 more

8 mentions without citation

REMO: A new protocol to refine full atomic protein models from C-alpha traces by optimizing hydrogen-bonding networks.
Li et al. (2009)
7 more