› 1nfd

X-ray diffraction
2.8Å resolution

AN ALPHA-BETA T CELL RECEPTOR (TCR) HETERODIMER IN COMPLEX WITH AN ANTI-TCR FAB FRAGMENT DERIVED FROM A MITOGENIC ANTIBODY

Released:
DOI: 10.2210/pdb1nfd/pdb
PDB entry 1nfd coloured by chain, front view.
PDB entry 1nfd coloured by chain, side view.
PDB entry 1nfd coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Atomic structure of an alphabeta T cell receptor (TCR) heterodimer in complex with an anti-TCR fab fragment derived from a mitogenic antibody.
Wang J, Lim K, Smolyar A, Teng M, Liu J, Tse AG, Liu J, Hussey RE, Chishti Y, Thomson CT, Sweet RM, Nathenson SG, Chang HC, Sacchettini JC, Reinherz EL
EMBO J 17 10-26 (1998)
PMID: 9427737

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-224982 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
T-cell receptor alpha chain constant Chains: A, C
Molecule details ›
Chains: A, C
Length: 203 amino acids
Theoretical weight: 22.61 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01849 (Residues: 1-90; Coverage: 66%)
Gene name: Trac
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T-cell receptor beta-1 chain C region Chains: B, D
Molecule details ›
Chains: B, D
Length: 239 amino acids
Theoretical weight: 27.31 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01852 (Residues: 1-127; Coverage: 73%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
H57 FAB Chains: E, G
Molecule details ›
Chains: E, G
Length: 212 amino acids
Theoretical weight: 23.05 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
H57 FAB Chains: F, H
Molecule details ›
Chains: F, H
Length: 222 amino acids
Theoretical weight: 24.3 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 14 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 74.74Å b: 122.3Å c: 115.84Å
α: 90° β: 107.95° γ: 90°
R-values:
R R work R free
0.243 0.243 0.309
Expression system: Cricetulus griseus

Quick links

Citations

25 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
24 more

40 mentions without citation

The structural basis of αβ T-lineage immune recognition: TCR docking topologies, mechanotransduction, and co-receptor function.
Wang et al. (2012)
39 more