› 1ni0

X-ray diffraction
2.5Å resolution

Structure of the Y94F mutant of the restriction endonuclease PvuII

Released:
DOI: 10.2210/pdb1ni0/pdb
PDB entry 1ni0 coloured by chain, front view.
PDB entry 1ni0 coloured by chain, side view.
PDB entry 1ni0 coloured by chain, top view.
Source organism: Proteus vulgaris
Entry authors: Tucker PA, Kokkinidis M, Nicolaki S, Kotsifaki D

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme PvuII Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 159 amino acids
Theoretical weight: 18.5 KDa
Source organism: Proteus vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: P23657 (Residues: 1-157; Coverage: 100%)
Gene name: pvuIIR
Sequence domains: Restriction endonuclease PvuII
Structure domains: PVUII Endonuclease, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P4212
Unit cell:
a: 176.86Å b: 176.86Å c: 46.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.29
Expression system: Escherichia coli

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