› 1nkt

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF THE SECA PROTEIN TRANSLOCATION ATPASE FROM MYCOBACTERIUM TUBERCULOSIS COMPLEX WITH ADPBS

Released:
DOI: 10.2210/pdb1nkt/pdb
PDB entry 1nkt coloured by chain, front view.
PDB entry 1nkt coloured by chain, side view.
PDB entry 1nkt coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of Mycobacterium tuberculosis SecA, a preprotein translocating ATPase.
Sharma V, Arockiasamy A, Ronning DR, Savva CG, Holzenburg A, Braunstein M, Jacobs WR, Sacchettini JC
Proc Natl Acad Sci U S A 100 2243-8 (2003)
PMID: 12606717

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-161506 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein translocase subunit SecA 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 922 amino acids
Theoretical weight: 103.46 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WGP5 (Residues: 2-892; Coverage: 94%)
Gene names: MTCY20B11.15c, Rv3240c, secA1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, APS BEAMLINE 14-BM-D
Spacegroup: P6222
Unit cell:
a: 206.05Å b: 206.05Å c: 292.857Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.213 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

36 review citations

The DEAD-box protein family of RNA helicases.
Cordin et al. (2006)
35 more

9 mentions without citation

SecA: a potential antimicrobial target.
Chaudhary et al. (2015)
8 more