› 1nmc

X-ray diffraction
2.5Å resolution

COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 15 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE

Released:
DOI: 10.2210/pdb1nmc/pdb
PDB entry 1nmc coloured by chain, front view.
PDB entry 1nmc coloured by chain, side view.
PDB entry 1nmc coloured by chain, top view.
Source organisms:
Primary publication:
Three-dimensional structures of single-chain Fv-neuraminidase complexes.
Malby RL, McCoy AJ, Kortt AA, Hudson PJ, Colman PM
J Mol Biol 279 901-10 (1998)
PMID: 9642070

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225023 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Neuraminidase Chains: A, N
Molecule details ›
Chains: A, N
Length: 388 amino acids
Theoretical weight: 43.72 KDa
Source organism: Influenza A virus
Expression system: Gallus gallus
UniProt:
  • Canonical: P03472 (Residues: 83-470; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase
SINGLE CHAIN ANTIBODY Chains: B, H
Molecule details ›
Chains: B, H
Length: 122 amino acids
Theoretical weight: 13.4 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
SINGLE CHAIN ANTIBODY Chains: C, L
Molecule details ›
Chains: C, L
Length: 109 amino acids
Theoretical weight: 12.16 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand MAN 2 x MAN
Ligand NAG 4 x NAG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P4212
Unit cell:
a: 144.4Å b: 144.4Å c: 227Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.26
Expression systems:
  • Gallus gallus
  • Escherichia coli

Quick links

Citations

4 review citations

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
3 more

6 mentions without citation

Reliable B cell epitope predictions: impacts of method development and improved benchmarking.
Kringelum et al. (2012)
5 more