› 1nou

X-ray diffraction
2.4Å resolution

Native human lysosomal beta-hexosaminidase isoform B

Released:
DOI: 10.2210/pdb1nou/pdb
PDB entry 1nou coloured by chain, front view.
PDB entry 1nou coloured by chain, side view.
PDB entry 1nou coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human beta-hexosaminidase B: understanding the molecular basis of Sandhoff and Tay-Sachs disease.
Mark BL, Mahuran DJ, Cherney MM, Zhao D, Knapp S, James MN
J Mol Biol 327 1093-109 (2003)
PMID: 12662933

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-hexosaminidase subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 507 amino acids
Theoretical weight: 58.18 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07686 (Residues: 50-556; Coverage: 99%)
Gene names: HCC7, HEXB
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6122
Unit cell:
a: 112.465Å b: 112.465Å c: 397.867Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.231

Quick links

Citations

11 review citations

The cell biology of lysosomal storage disorders.
Futerman et al. (2004)
10 more

11 mentions without citation

A mechanical explanation of RNA pseudoknot function in programmed ribosomal frameshifting.
Namy et al. (2006)
10 more