› 1npq

Solution NMR

structure of a rhodamine-labeled N-domain Troponin C mutant (Ca2+ saturated) in complex with skeletal Troponin I 115-131

Released:
DOI: 10.2210/pdb1npq/pdb
PDB entry 1npq coloured by chain, ensemble of 21 models, front view.
PDB entry 1npq coloured by chain, ensemble of 21 models, side view.
PDB entry 1npq coloured by chain, ensemble of 21 models, top view.
Source organisms:
Primary publication:
NMR structure of a bifunctional rhodamine labeled N-domain of troponin C complexed with the regulatory "switch" peptide from troponin I: implications for in situ fluorescence studies in muscle fibers.
Mercier P, Ferguson RE, Irving M, Corrie JE, Trentham DR, Sykes BD
Biochemistry 42 4333-48 (2003)
PMID: 12693929

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135942 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Troponin C, skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 9.93 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02588 (Residues: 2-91; Coverage: 55%)
Gene name: TNNC2
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Troponin I, fast skeletal muscle Chain: B
Molecule details ›
Chain: B
Length: 17 amino acids
Theoretical weight: 1.89 KDa
Source organism: Oryctolagus cuniculus
Expression system: Not provided
UniProt:
  • Canonical: P02643 (Residues: 116-132; Coverage: 9%)
Gene name: TNNI2

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing using torsion angle dynamics AND cartesian dynamics with the program CNS 1.1
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Structural based insights into the role of troponin in cardiac muscle pathophysiology.
Li et al. (2004)
4 more