› 1npr

X-ray diffraction
2.21Å resolution

CRYSTAL STRUCTURE OF AQUIFEX AEOLICUS NUSG IN C222(1)

Released:
DOI: 10.2210/pdb1npr/pdb
PDB entry 1npr coloured by chain, front view.
PDB entry 1npr coloured by chain, side view.
PDB entry 1npr coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
A spring-loaded state of NusG in its functional cycle is suggested by X-ray crystallography and supported by site-directed mutants.
Knowlton JR, Bubunenko M, Andrykovitch M, Guo W, Routzahn KM, Waugh DS, Court DL, Ji X
Biochemistry 42 2275-81 (2003)
PMID: 12600194

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination/antitermination protein NusG Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 28.04 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67757 (Residues: 1-248; Coverage: 100%)
Gene names: aq_1931, nusG
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 65.95Å b: 124.58Å c: 83.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

NusG-Spt5 proteins-Universal tools for transcription modification and communication.
Tomar et al. (2013)
31 other articles

2 mentions without citation

A nexus for gene expression-molecular mechanisms of Spt5 and NusG in the three domains of life.
Werner F. (2012)
1 more