› 1nrg

X-ray diffraction
1.95Å resolution

Structure and Properties of Recombinant Human Pyridoxine-5'-Phosphate Oxidase

Released:
DOI: 10.2210/pdb1nrg/pdb
PDB entry 1nrg coloured by chain, front view.
PDB entry 1nrg coloured by chain, side view.
PDB entry 1nrg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase.
Musayev FN, Di Salvo ML, Ko TP, Schirch V, Safo MK
Protein Sci 12 1455-63 (2003)
PMID: 12824491

Function and Biology Details

Reaction catalysed: 
Pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'-phosphate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxine-5'-phosphate oxidase Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 30.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NVS9 (Residues: 1-261; Coverage: 100%)
Gene name: PNPO
Sequence domains:
Structure domains: Electron Transport, Fmn-binding Protein; Chain A

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
3 bound ligands:
Ligand PO4 1 x PO4
Ligand PLP 1 x PLP
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 82.747Å b: 82.747Å c: 59.327Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.192 0.253
Expression system: Escherichia coli

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Citations

6 review citations

Vitamin B6: a long known compound of surprising complexity.
Mooney et al. (2009)
5 more

19 mentions without citation

The human flavoproteome.
Lienhart et al. (2013)
18 more