› 1nsa

X-ray diffraction
2.3Å resolution

THREE-DIMENSIONAL STRUCTURE OF PORCINE PROCARBOXYPEPTIDASE B: A STRUCTURAL BASIS OF ITS INACTIVITY

Released:
DOI: 10.2210/pdb1nsa/pdb
PDB entry 1nsa coloured by chain, front view.
PDB entry 1nsa coloured by chain, side view.
PDB entry 1nsa coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Three-dimensional structure of porcine procarboxypeptidase B: a structural basis of its inactivity.
Coll M, Guasch A, Avilés FX, Huber R
EMBO J 10 1-9 (1991)
PMID: 1989878

Function and Biology Details

Reaction catalysed: 
Preferential release of a C-terminal lysine or arginine amino acid.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxypeptidase B Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 45 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P09955 (Residues: 22-416; Coverage: 99%)
Gene names: CPB, CPB1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand BEN 3 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43
Unit cell:
a: 103.1Å b: 103.1Å c: 46.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 not available

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Citations

13 review citations

Natural protein proteinase inhibitors and their interaction with proteinases.
Bode et al. (1992)
12 more

5 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
4 more