› 1nsf

X-ray diffraction
1.9Å resolution

D2 HEXAMERIZATION DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)

Released:
DOI: 10.2210/pdb1nsf/pdb
PDB entry 1nsf coloured by chain, front view.
PDB entry 1nsf coloured by chain, side view.
PDB entry 1nsf coloured by chain, top view.
Source organism: Cricetulus griseus
Primary publication:
Structure of the ATP-dependent oligomerization domain of N-ethylmaleimide sensitive factor complexed with ATP.
Yu RC, Hanson PI, Jahn R, Brünger AT
Nat Struct Biol 5 803-11 (1998)
PMID: 9731775

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vesicle-fusing ATPase Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 30.49 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18708 (Residues: 478-744; Coverage: 36%)
Gene name: NSF
Sequence domains: ATPase family associated with various cellular activities (AAA)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P6
Unit cell:
a: 115.996Å b: 115.996Å c: 44.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.224 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

49 review citations

The 26S proteasome: a molecular machine designed for controlled proteolysis.
Voges et al. (1999)
48 more

24 mentions without citation

Mechanism of neurotransmitter release coming into focus.
Rizo J. (2018)
23 more