› 1ntg

X-ray diffraction
2.21Å resolution

Crystal Structure of the EMAP II-like Cytokine Released from human tyrosyl-tRNA Synthetase

Released:
DOI: 10.2210/pdb1ntg/pdb
PDB entry 1ntg coloured by chain, front view.
PDB entry 1ntg coloured by chain, side view.
PDB entry 1ntg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of an EMAP-II-like Cytokine Released from a human tRNA Synthetase
Yang X-L, Liu J, Skene RJ, McRee DE, Schimmel P
Helv. Chim. Acta 86 1246-1257 (2003)

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157046 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 172 amino acids
Theoretical weight: 19.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P54577 (Residues: 359-528; Coverage: 32%)
Gene names: YARS, YARS1
Sequence domains: Putative tRNA binding domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P1
Unit cell:
a: 54.621Å b: 59.515Å c: 71.591Å
α: 103.51° β: 109.95° γ: 101.52°
R-values:
R R work R free
0.251 0.251 0.296
Expression system: Escherichia coli

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Citations

5 mentions without citation

Functional expansion of human tRNA synthetases achieved by structural inventions.
Guo et al. (2010)
4 more