› 1nu5

X-ray diffraction
1.95Å resolution

Crystal structure of Pseudomonas sp. P51 Chloromuconate lactonizing enzyme

Released:
DOI: 10.2210/pdb1nu5/pdb
PDB entry 1nu5 coloured by chain, front view.
PDB entry 1nu5 coloured by chain, side view.
PDB entry 1nu5 coloured by chain, top view.
Source organism: Pseudomonas sp. P51
Primary publication:
The structure of Pseudomonas P51 Cl-muconate lactonizing enzyme: co-evolution of structure and dynamics with the dehalogenation function.
Kajander T, Lehtiö L, Schlömann M, Goldman A
Protein Sci 12 1855-64 (2003)
PMID: 12930985

Function and Biology Details

Reaction catalysed: 
(2R)-2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloromuconate cycloisomerase Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 39.53 KDa
Source organism: Pseudomonas sp. P51
Expression system: Escherichia coli
UniProt:
  • Canonical: P27099 (Residues: 1-370; Coverage: 100%)
Gene name: tcbD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: I422
Unit cell:
a: 135.355Å b: 135.355Å c: 105.033Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.231
Expression system: Escherichia coli

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Citations

2 review citations

Divergent evolution in the enolase superfamily: the interplay of mechanism and specificity.
Gerlt et al. (2005)
1 more

5 mentions without citation

Discordant and chameleon sequences: their distribution and implications for amyloidogenicity.
Gendoo et al. (2011)
4 more