› 1nug

X-ray diffraction
2.4Å resolution

Role of Calcium Ions in the Activation and Activity of the Transglutaminase 3 Enzyme (2 calciums, 1 Mg, inactive form)

Released:
DOI: 10.2210/pdb1nug/pdb
PDB entry 1nug coloured by chain, front view.
PDB entry 1nug coloured by chain, side view.
PDB entry 1nug coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Roles of calcium ions in the activation and activity of the transglutaminase 3 enzyme.
Ahvazi B, Boeshans KM, Idler W, Baxa U, Steinert PM
J Biol Chem 278 23834-41 (2003)
PMID: 12679341

Function and Biology Details

Reaction catalysed: 
A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein-glutamine gamma-glutamyltransferase E Chains: A, B
Molecule details ›
Chains: A, B
Length: 692 amino acids
Theoretical weight: 76.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q08188 (Residues: 2-693; Coverage: 100%)
Gene name: TGM3
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 4 x CL
Ligand CA 4 x CA
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P1
Unit cell:
a: 57.51Å b: 66.81Å c: 116.53Å
α: 97.24° β: 90.28° γ: 98.36°
R-values:
R R work R free
0.194 0.194 0.248
Expression system: Spodoptera frugiperda

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Citations

11 review citations

Oxidative stress in aging human skin.
Rinnerthaler et al. (2015)
10 more

4 mentions without citation

The diamond anniversary of tissue transglutaminase: a protein of many talents.
Katt et al. (2018)
3 more