› 1nuh

X-ray diffraction
2.51Å resolution

The crystal structure of human phosphoglucose isomerase complexed with 5-phosphoarabinonate

Released:
DOI: 10.2210/pdb1nuh/pdb
PDB entry 1nuh coloured by chain, front view.
PDB entry 1nuh coloured by chain, side view.
PDB entry 1nuh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human phosphoglucose isomerase complexed with a transition-state analogue.
Davies C, Muirhead H, Chirgwin J
Acta Crystallogr D Biol Crystallogr 59 1111-3 (2003)
PMID: 12777791

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chain: A
Molecule details ›
Chain: A
Length: 558 amino acids
Theoretical weight: 63.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06744 (Residues: 1-558; Coverage: 100%)
Gene name: GPI
Sequence domains: Phosphoglucose isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand PA5 1 x PA5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P43212
Unit cell:
a: 94.4Å b: 94.4Å c: 137.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.268
Expression system: Escherichia coli BL21

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Citations

1 review citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
13 other articles

4 mentions without citation

Betulinic Acid Exerts Cytotoxic Activity Against Multidrug-Resistant Tumor Cells via Targeting Autocrine Motility Factor Receptor (AMFR).
Saeed et al. (2018)
3 more