› 1nuv

X-ray diffraction
1.81Å resolution

The Leadzyme Ribozyme Bound to Mg(H2O)6(II) and Sr(II) at 1.8 A resolution

Released:
DOI: 10.2210/pdb1nuv/pdb
PDB entry 1nuv coloured by chain, front view.
PDB entry 1nuv coloured by chain, side view.
PDB entry 1nuv coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structure of the leadzyme at 1.8 A resolution: metal ion binding and the implications for catalytic mechanism and allo site ion regulation.
Wedekind JE, McKay DB
Biochemistry 42 9554-63 (2003)
PMID: 12911297

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196146 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
5'-R(*CP*GP*GP*AP*CP*CP*GP*AP*GP*CP*CP*AP*G)-3' Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 13 nucleotides
Theoretical weight: 4.19 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(*GP*CP*UP*GP*GP*GP*AP*GP*UP*CP*C)-3' Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 11 nucleotides
Theoretical weight: 3.54 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 10 x MG
Ligand SR 6 x SR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P61
Unit cell:
a: 60.6Å b: 60.6Å c: 133.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.21 0.219
Expression system: Not provided

Quick links

Citations

4 review citations

Nucleic acid crystallography: current progress.
Egli M. (2004)
3 more

2 mentions without citation

Crystal structure of an RNA-cleaving DNAzyme.
Liu et al. (2017)
1 more