› 1nxz

X-ray diffraction
2Å resolution

X-Ray Crystal Structure of Protein yggj_haein of Haemophilus influenzae. Northeast Structural Genomics Consortium Target IR73.

Released:
DOI: 10.2210/pdb1nxz/pdb
PDB entry 1nxz coloured by chain, front view.
PDB entry 1nxz coloured by chain, side view.
PDB entry 1nxz coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Functional assignment based on structural analysis: crystal structure of the yggJ protein (HI0303) of Haemophilus influenzae reveals an RNA methyltransferase with a deep trefoil knot.
Forouhar F, Shen J, Xiao R, Acton TB, Montelione GT, Tong L
Proteins 53 329-32 (2003)
PMID: 14517985

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase E Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.61 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P44627 (Residues: 2-245; Coverage: 100%)
Gene names: HI_0303, rsmE
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 61.305Å b: 73.517Å c: 109.471Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.214 0.264
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structures and folding pathways of topologically knotted proteins.
Virnau et al. (2011)
3 more

6 mentions without citation

Structural and evolutionary bioinformatics of the SPOUT superfamily of methyltransferases.
Tkaczuk et al. (2007)
5 more