› 1obo

X-ray diffraction
1.2Å resolution

W57L flavodoxin from Anabaena

Released:
DOI: 10.2210/pdb1obo/pdb
PDB entry 1obo coloured by chain, front view.
PDB entry 1obo coloured by chain, side view.
PDB entry 1obo coloured by chain, top view.
Source organism: Nostoc sp. PCC 7119
Primary publication:
How FMN binds to anabaena apoflavodoxin: a hydrophobic encounter at an open binding site.
Lostao A, Daoudi F, Irún MP, Ramon A, Fernández-Cabrera C, Romero A, Sancho J
J Biol Chem 278 24053-61 (2003)
PMID: 12682068

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavodoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 18.76 KDa
Source organism: Nostoc sp. PCC 7119
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A3E0 (Residues: 2-170; Coverage: 99%)
Gene name: isiB
Sequence domains: Flavodoxin
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand FMN 2 x FMN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 37.63Å b: 73.1Å c: 57.05Å
α: 90° β: 102.58° γ: 90°
R-values:
R R work R free
0.186 0.186 0.202
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Folding of proteins with a flavodoxin-like architecture.
Houwman et al. (2017)
17 other articles

5 mentions without citation

3(10) helices in channels and other membrane proteins.
Vieira-Pires et al. (2010)
4 more