› 1oct

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF THE OCT-1 POU DOMAIN BOUND TO AN OCTAMER SITE: DNA RECOGNITION WITH TETHERED DNA-BINDING MODULES

Released:
DOI: 10.2210/pdb1oct/pdb
PDB entry 1oct coloured by chain, front view.
PDB entry 1oct coloured by chain, side view.
PDB entry 1oct coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the Oct-1 POU domain bound to an octamer site: DNA recognition with tethered DNA-binding modules.
Klemm JD, Rould MA, Aurora R, Herr W, Pabo CO
Cell 77 21-32 (1994)
PMID: 8156594

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114300 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
POU domain, class 2, transcription factor 1 Chain: C
Molecule details ›
Chain: C
Length: 156 amino acids
Theoretical weight: 18.01 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P14859 (Residues: 284-439; Coverage: 21%)
Gene names: OCT1, OTF1, POU2F1
Sequence domains:
Structure domains:
DNA (5'-D(*TP*GP*TP*AP*TP*GP*CP*AP*AP*AP*TP*AP*AP*GP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 15 nucleotides
Theoretical weight: 4.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*CP*CP*TP*TP*AP*TP*TP*TP*GP*CP*AP*TP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.52 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 97.5Å b: 89.8Å c: 80Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 not available
Expression system: Not provided

Quick links

Citations

51 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
50 more

26 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
25 more