1oe6

X-ray diffraction
2.65Å resolution

Xenopus SMUG1, an anti-mutator uracil-DNA Glycosylase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-114305 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Single-strand selective monofunctional uracil DNA glycosylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 27.75 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9YGN6 (Residues: 35-281; Coverage: 88%)
Gene name: smug1
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain
5'-D(*CP*CP*CP*GP*TP*GP*AP*GP*TP*CP*CP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 12 nucleotides
Theoretical weight: 3.64 KDa
Source organism: Xenopus laevis
Expression system: Not provided
5'-D(*CP*GP*GP*AP*CP*TP*3DRP*AP*CP*GP*GP*G)-3' Chain: F
Molecule details ›
Chain: F
Length: 12 nucleotides
Theoretical weight: 3.58 KDa
Source organism: Xenopus laevis
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 120.969Å b: 86.518Å c: 78.456Å
α: 90° β: 118.53° γ: 90°
R-values:
R R work R free
0.157 0.154 0.218
Expression systems:
  • Escherichia coli
  • Not provided