› 1oe9

X-ray diffraction
2.05Å resolution

Crystal structure of Myosin V motor with essential light chain-nucleotide-free

Released:
DOI: 10.2210/pdb1oe9/pdb
PDB entry 1oe9 coloured by chain, front view.
PDB entry 1oe9 coloured by chain, side view.
PDB entry 1oe9 coloured by chain, top view.
Source organisms:
Primary publication:
A structural state of the myosin V motor without bound nucleotide.
Coureux PD, Wells AL, Ménétrey J, Yengo CM, Morris CA, Sweeney HL, Houdusse A
Nature 425 419-23 (2003)
PMID: 14508494

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147010 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Unconventional myosin-Va Chain: A
Molecule details ›
Chain: A
Length: 795 amino acids
Theoretical weight: 91.61 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02440 (Residues: 1-792; Coverage: 43%)
Gene name: MYO5A
Sequence domains:
Myosin light chain 6B Chain: B
Molecule details ›
Chain: B
Length: 151 amino acids
Theoretical weight: 17.09 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14649 (Residues: 58-208; Coverage: 73%)
Gene names: MLC1SA, MYL6B
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 53.933Å b: 98.247Å c: 111.378Å
α: 90° β: 101.43° γ: 90°
R-values:
R R work R free
0.222 0.22 0.264
Expression system: Spodoptera frugiperda

Quick links

Citations

35 review citations

Structural and functional insights into the Myosin motor mechanism.
Sweeney et al. (2010)
34 more

51 mentions without citation

Myosin V from head to tail.
Trybus KM. (2008)
50 more