› 1ofc

X-ray diffraction
1.9Å resolution

nucleosome recognition module of ISWI ATPase

Released:
DOI: 10.2210/pdb1ofc/pdb
PDB entry 1ofc coloured by chain, front view.
PDB entry 1ofc coloured by chain, side view.
PDB entry 1ofc coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure and functional analysis of a nucleosome recognition module of the remodeling factor ISWI.
Grüne T, Brzeski J, Eberharter A, Clapier CR, Corona DF, Becker PB, Müller CW
Mol Cell 12 449-60 (2003)
PMID: 14536084

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromatin-remodeling complex ATPase chain Iswi Chain: X
Molecule details ›
Chain: X
Length: 304 amino acids
Theoretical weight: 35.89 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q24368 (Residues: 691-991; Coverage: 29%)
Gene names: CG8625, Iswi
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLC 1 x GLC
Ligand G4D 1 x G4D
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 109.47Å b: 66.34Å c: 82.83Å
α: 90° β: 124.41° γ: 90°
R-values:
R R work R free
0.219 0.218 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

58 review citations

The biology of chromatin remodeling complexes.
Clapier et al. (2009)
57 more

10 mentions without citation

Domain architecture of the catalytic subunit in the ISW2-nucleosome complex.
Dang et al. (2007)
9 more