› 1ofx

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1ofx/pdb
PDB entry 1ofx coloured by chain, front view.
PDB entry 1ofx coloured by chain, side view.
PDB entry 1ofx coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structure of an Okazaki fragment at 2-A resolution.
Egli M, Usman N, Zhang SG, Rich A
Proc Natl Acad Sci U S A 89 534-8 (1992)
PMID: 1370582

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-113087 (preferred)
Entry contents:
1 distinct DNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
DNA (5'-D(*GP*GP*GP*TP*AP*TP*AP*CP*GP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA/RNA (5'-R(*GP*CP*GP*)-D(*TP*AP*TP*AP*CP*CP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SPM 1 x SPM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 24.03Å b: 43.67Å c: 48.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 not available not available
Expression system: Not provided

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Citations

9 review citations

Recognition of the unique structure of DNA:RNA hybrids.
Shaw et al. (2008)
8 more