› 1oh7

X-ray diffraction
2.5Å resolution

THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:G MISMATCH

Released:
DOI: 10.2210/pdb1oh7/pdb
PDB entry 1oh7 coloured by chain, front view.
PDB entry 1oh7 coloured by chain, side view.
PDB entry 1oh7 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of Escherichia coli DNA mismatch repair enzyme MutS in complex with different mismatches: a common recognition mode for diverse substrates.
Natrajan G, Lamers MH, Enzlin JH, Winterwerp HH, Perrakis A, Sixma TK
Nucleic Acids Res 31 4814-21 (2003)
PMID: 12907723

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114308 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 800 amino acids
Theoretical weight: 89.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P23909 (Residues: 1-800; Coverage: 94%)
Gene names: JW2703, b2733, fdv, mutS
Sequence domains:
Structure domains:
5'-D(*AP*GP*CP*TP*GP*CP*CP*AP*GP*GP *CP*AP*CP*CP*AP*GP*TP*GP*TP*CP*AP*GP*CP*GP*TP *CP*CP*TP*AP*T)-3' Chain: E
Molecule details ›
Chain: E
Length: 30 nucleotides
Theoretical weight: 9.18 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*AP*TP*AP*GP*GP*AP*CP*GP*CP*TP *GP*AP*CP*AP*CP*TP*GP*GP*TP*GP*CP*GP*TP*GP*GP *CP*AP*GP*CP*T)-3' Chain: F
Molecule details ›
Chain: F
Length: 30 nucleotides
Theoretical weight: 9.3 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 89.413Å b: 91.81Å c: 260.444Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

22 review citations

DNA mismatch repair.
Kunkel et al. (2005)
21 more

5 mentions without citation

Structures of Escherichia coli DNA mismatch repair enzyme MutS in complex with different mismatches: a common recognition mode for diverse substrates.
Natrajan et al. (2003)
4 more