› 1ohq

X-ray diffraction
2Å resolution

Crystal structure of HEL4, a soluble human VH antibody domain resistant to aggregation

Released:
DOI: 10.2210/pdb1ohq/pdb
PDB entry 1ohq coloured by chain, front view.
PDB entry 1ohq coloured by chain, side view.
PDB entry 1ohq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of HEL4, a soluble, refoldable human V(H) single domain with a germ-line scaffold.
Jespers L, Schon O, James LC, Veprintsev D, Winter G
J Mol Biol 337 893-903 (2004)
PMID: 15033359

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134595 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin heavy variable 3-23 Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 12.86 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P01764 (Residues: 20-116; Coverage: 99%)
Gene name: IGHV3-23
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: I4122
Unit cell:
a: 76.121Å b: 76.121Å c: 187.069Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.26
Expression system: Komagataella pastoris

Quick links

Citations

19 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
18 more

8 mentions without citation

Single-Domain Antibodies As Therapeutics against Human Viral Diseases.
Wu et al. (2017)
7 more