› 1oix

X-ray diffraction
1.7Å resolution

X-ray structure of the small G protein Rab11a in complex with GDP and Pi

Released:
DOI: 10.2210/pdb1oix/pdb
PDB entry 1oix coloured by chain, front view.
PDB entry 1oix coloured by chain, side view.
PDB entry 1oix coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic evidence for substrate-assisted GTP hydrolysis by a small GTP binding protein.
Pasqualato S, Cherfils J
Structure 13 533-40 (2005)
PMID: 15837192

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-11A Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62491 (Residues: 1-173; Coverage: 80%)
Gene names: RAB11, RAB11A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand GDP 1 x GDP
Ligand PO4 1 x PO4
Ligand MG 1 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I422
Unit cell:
a: 74.077Å b: 74.077Å c: 124.905Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.229
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
6 more

5 mentions without citation

Structural basis of myosin V Rab GTPase-dependent cargo recognition.
Pylypenko et al. (2013)
4 more