› 1oj4

X-ray diffraction
2.01Å resolution

Ternary complex of 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Released:
DOI: 10.2210/pdb1oj4/pdb
PDB entry 1oj4 coloured by chain, front view.
PDB entry 1oj4 coloured by chain, side view.
PDB entry 1oj4 coloured by chain, top view.
Source organism: Escherichia coli O6
Primary publication:
Biosynthesis of isoprenoids: crystal structure of 4-diphosphocytidyl-2C-methyl-D-erythritol kinase.
Miallau L, Alphey MS, Kemp LE, Leonard GA, McSweeney SM, Hecht S, Bacher A, Eisenreich W, Rohdich F, Hunter WN
Proc Natl Acad Sci U S A 100 9173-8 (2003)
PMID: 12878729

Function and Biology Details

Reaction catalysed: 
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 31.27 KDa
Source organism: Escherichia coli O6
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8FI04 (Residues: 1-283; Coverage: 100%)
Gene names: c1666, ispE
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CDM 2 x CDM
Ligand ANP 2 x ANP
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 61Å b: 76.3Å c: 68.6Å
α: 90° β: 107.8° γ: 90°
R-values:
R R work R free
0.167 0.166 0.202
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Molecular recognition in chemical and biological systems.
Persch et al. (2015)
11 more

5 mentions without citation

Mechanistic aspects of carotenoid biosynthesis.
Moise et al. (2014)
4 more