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› 1one

X-ray diffraction
1.8Å resolution

YEAST ENOLASE COMPLEXED WITH AN EQUILIBRIUM MIXTURE OF 2'-PHOSPHOGLYCEATE AND PHOSPHOENOLPYRUVATE

Released:
DOI: 10.2210/pdb1one/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1one coloured by chain, front view.
PDB entry 1one coloured by chain, side view.
PDB entry 1one coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A carboxylate oxygen of the substrate bridges the magnesium ions at the active site of enolase: structure of the yeast enzyme complexed with the equilibrium mixture of 2-phosphoglycerate and phosphoenolpyruvate at 1.8 A resolution.
Larsen TM, Wedekind JE, Rayment I, Reed GH
Biochemistry 35 4349-58 (1996)
PMID: 8605183

Function and Biology Details

Reaction catalysed: 
(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 46.73 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00924 (Residues: 2-437; Coverage: 100%)
Gene names: ENO1, ENOA, G9160, HSP48, YGR254W
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand PEP 2 x PEP
Ligand 2PG 2 x 2PG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
Spacegroup: C2
Unit cell:
a: 121.9Å b: 73.2Å c: 93.9Å
α: 90° β: 93.3° γ: 90°
R-values:
R R work R free
0.177 0.177 not available

Quick links

Citations

9 review citations

The lipocalin protein family: structural and sequence overview.
Flower et al. (2000)
8 more

14 mentions without citation

Targeting Unconventional Pathways in Pursuit of Novel Antifungals.
Nguyen et al. (2020)
13 more