› 1opj

X-ray diffraction
1.75Å resolution

Structural basis for the auto-inhibition of c-Abl tyrosine kinase

Released:
DOI: 10.2210/pdb1opj/pdb
PDB entry 1opj coloured by chain, front view.
PDB entry 1opj coloured by chain, side view.
PDB entry 1opj coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for the autoinhibition of c-Abl tyrosine kinase.
Nagar B, Hantschel O, Young MA, Scheffzek K, Veach D, Bornmann W, Clarkson B, Superti-Furga G, Kuriyan J
Cell 112 859-71 (2003)
PMID: 12654251

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ABL1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 293 amino acids
Theoretical weight: 33.74 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00520 (Residues: 229-515; Coverage: 26%)
Gene names: Abl, Abl1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand MYR 2 x MYR
Ligand STI 2 x STI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 41.844Å b: 63.507Å c: 64.051Å
α: 67.91° β: 79.76° γ: 84.88°
R-values:
R R work R free
0.21 0.21 0.242
Expression system: Spodoptera frugiperda

Quick links

Citations

112 review citations

Targeting cancer with small molecule kinase inhibitors.
Zhang et al. (2009)
111 more

46 mentions without citation

Protein kinases: evolution of dynamic regulatory proteins.
Taylor et al. (2011)
45 more