› 1oqv

X-ray diffraction
1.3Å resolution

Structure of TcpA, the Type IV pilin subunit from the toxin co-regulated pilus of Vibrio cholerae classical biotype

Released:
DOI: 10.2210/pdb1oqv/pdb
PDB entry 1oqv coloured by chain, front view.
PDB entry 1oqv coloured by chain, side view.
PDB entry 1oqv coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Type IV pilin structure and assembly: X-ray and EM analyses of Vibrio cholerae toxin-coregulated pilus and Pseudomonas aeruginosa PAK pilin.
Craig L, Taylor RK, Pique ME, Adair BD, Arvai AS, Singh M, Lloyd SJ, Shin DS, Getzoff ED, Yeager M, Forest KT, Tainer JA
Mol Cell 11 1139-50 (2003)
PMID: 12769840

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149867 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toxin coregulated pilin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 192 amino acids
Theoretical weight: 19.79 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: P23024 (Residues: 54-224; Coverage: 76%)
Gene name: tcpA
Sequence domains: Toxin-coregulated pilus subunit TcpA
Structure domains: TcpA-like pilin

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P63
Unit cell:
a: 157.317Å b: 157.317Å c: 35.719Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.117 0.116 0.174
Expression system: Escherichia coli

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Citations

32 review citations

Type IV pilus structure and bacterial pathogenicity.
Craig et al. (2004)
31 more

15 mentions without citation

Type IV pilin proteins: versatile molecular modules.
Giltner et al. (2012)
14 more