› 1os4

X-ray diffraction
2.25Å resolution

Dehydrated T6 human insulin at 295 K

Released:
DOI: 10.2210/pdb1os4/pdb
PDB entry 1os4 coloured by chain, front view.
PDB entry 1os4 coloured by chain, side view.
PDB entry 1os4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Lessons from an aged, dried crystal of T(6) human insulin.
Smith GD, Blessing RH
Acta Crystallogr D Biol Crystallogr 59 1384-94 (2003)
PMID: 12876340

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 33.9Å b: 49.44Å c: 49.26Å
α: 115.12° β: 104.01° γ: 102.69°
R-values:
R R work R free
0.243 0.243 0.295

Quick links

Citations

2 review citations

Thermodynamic and dynamic factors involved in the stability of native protein structure in amorphous solids in relation to levels of hydration.
Hill et al. (2005)
1 more