› 1os7

X-ray diffraction
2.5Å resolution

Crystal structure of TauD with iron, alpha-ketoglutarate and Taurine bound at pH 7.5

Released:
DOI: 10.2210/pdb1os7/pdb
PDB entry 1os7 coloured by chain, front view.
PDB entry 1os7 coloured by chain, side view.
PDB entry 1os7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Substrate-induced conformational changes in Escherichia coli taurine/alpha-ketoglutarate dioxygenase and insight into the oligomeric structure.
O'Brien JR, Schuller DJ, Yang VS, Dillard BD, Lanzilotta WN
Biochemistry 42 5547-54 (2003)
PMID: 12741810

Function and Biology Details

Reaction catalysed: 
Taurine + 2-oxoglutarate + O(2) = sulfite + aminoacetaldehyde + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-ketoglutarate-dependent taurine dioxygenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 283 amino acids
Theoretical weight: 32.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37610 (Residues: 1-283; Coverage: 100%)
Gene names: JW0360, b0368, ssiD, tauD, yaiG
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like

Ligands and Environments

3 bound ligands:
Ligand FE2 4 x FE2
Ligand TAU 3 x TAU
Ligand AKG 4 x AKG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 92.594Å b: 118.855Å c: 118.832Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.278
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins.
Clifton et al. (2006)
7 more

27 mentions without citation

Mechanisms of nickel toxicity in microorganisms.
Macomber et al. (2011)
26 more