› 1osg

X-ray diffraction
3Å resolution

Complex between BAFF and a BR3 derived peptide presented in a beta-hairpin scaffold

Released:
DOI: 10.2210/pdb1osg/pdb
PDB entry 1osg coloured by chain, front view.
PDB entry 1osg coloured by chain, side view.
PDB entry 1osg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
BAFF/BLyS receptor 3 comprises a minimal TNF receptor-like module that encodes a highly focused ligand-binding site.
Gordon NC, Pan B, Hymowitz SG, Yin J, Kelley RF, Cochran AG, Yan M, Dixit VM, Fairbrother WJ, Starovasnik MA
Biochemistry 42 5977-83 (2003)
PMID: 12755599

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162357 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor ligand superfamily member 13b, membrane form Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 208 amino acids
Theoretical weight: 22.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y275 (Residues: 82-285; Coverage: 72%)
Gene names: BAFF, BLYS, TALL1, TNFSF13B, TNFSF20, UNQ401/PRO738, ZTNF4
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
BR3 derived PEPTIDE Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 12 amino acids
Theoretical weight: 1.57 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P65
Unit cell:
a: 121.633Å b: 121.633Å c: 157.214Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.213 0.286
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

12 review citations

BAFF, APRIL and their receptors: structure, function and signaling.
Bossen et al. (2006)
11 more

2 mentions without citation

Exploiting structure similarity in refinement: automated NCS and target-structure restraints in BUSTER.
Smart et al. (2012)
1 more