Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
Lactose operon repressor and DNA (preferred)
PDBe Complex ID:
PDB-CPX-114323 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Lactose operon repressor
Molecule details ›
Chains: A, B
Length: 62 amino acids
Theoretical weight: 6.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Sequence domains: Bacterial regulatory proteins, lacI family
Structure domains: lambda repressor-like DNA-binding domains
Length: 62 amino acids
Theoretical weight: 6.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical:
P03023 (Residues: 1-62; Coverage: 17%)
Sequence domains: Bacterial regulatory proteins, lacI family
Structure domains: lambda repressor-like DNA-binding domains
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
THE STRUCTURE OF THE COMPLEX WAS CALCULATED AS FOLLOWS. FIRST THE STRUCTURE OF THE DIMERIC LACHP62-V52C WAS CALCULATED USING ONLY PROTEIN NMR RESTRAINTS. THE 100 BEST STRUCTURES WERE SELECTED AND DOCKED ONTO THE NONSPECIFIC LAC OPERATOR B-DNA USING SIMULATED ANNEALING. DISTANCE AND PLANARITY RESTRAINTS FOR THE DNA WERE INCORPORATED IN ORDER TO KEEP DNA CLOSE TO B-DNA CONFORMATION.
Expression systems:
- Escherichia coli
- Not provided
