› 1otg

X-ray diffraction
2.1Å resolution

5-CARBOXYMETHYL-2-HYDROXYMUCONATE ISOMERASE

Released:
DOI: 10.2210/pdb1otg/pdb
PDB entry 1otg coloured by chain, front view.
PDB entry 1otg coloured by chain, side view.
PDB entry 1otg coloured by chain, top view.
Source organism: Escherichia coli C
Primary publication:
Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases.
Subramanya HS, Roper DI, Dauter Z, Dodson EJ, Davies GJ, Wilson KS, Wigley DB
Biochemistry 35 792-802 (1996)
PMID: 8547259

Function and Biology Details

Reaction catalysed: 
5-carboxymethyl-2-hydroxymuconate = (3E,5R)-5-carboxy-2-oxohept-3-enedioate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-carboxymethyl-2-hydroxymuconate Delta-isomerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 125 amino acids
Theoretical weight: 14.16 KDa
Source organism: Escherichia coli C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05354 (Residues: 2-126; Coverage: 99%)
Gene name: hpcD
Sequence domains: 5-carboxymethyl-2-hydroxymuconate isomerase
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P41212
Unit cell:
a: 90.3Å b: 90.3Å c: 129.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 not available not available
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Macrophage migration inhibitory factor: a regulator of innate immunity.
Calandra et al. (2003)
17 more

1 mention without citation

Interaction preferences across protein-protein interfaces of obligatory and non-obligatory components are different.
De et al. (2005)